Sequence tags {adapter sequence} are in probes.
Single-stranded DNA sequences {oligonucleotide}| {oligo} can have less than 61 bases.
To add deoxyribonucleotides to nucleic acid by DNA polymerase requires oligonucleotides {primer, DNA}|.
Short RNAs or single-stranded DNAs {probe, DNA} {DNA probe} can detect complementary base sequences by hybridization. Probes have 25 to 60 bases and can have 3'-hexyl-amine. Probes attach to last 1500 base pairs closer to transcript 3' ends, where genes have unique short DNA regions. Bacteria and yeast genes have unique primers. Higher organisms have three million different expressed sequence tags (EST).
process
High-concentration purified probes are in 96, 384, or 1536 wells on plastic microtiter plates. Robots take probes from microtiter plate to make same number of spots on glass slides, one slide for each RNA sample to test. Multiple probes test each gene.
Nucleotide sequences {zipcode} can attach to molecules to allow probe complementary nucleotide sequence {zipcode complement} to hybridize.
4-Genetics-Recombinant DNA-Amplification
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Date Modified: 2022.0225